Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile
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- 作者：Huihui Sun ; Hualei Wang ; hlwang@mail.ecust.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Wenyuan Gao ; Lifeng Chen ; Kai Wu ; Dongzhi Wei ; dzhwei@ecust.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Directed evolution ; Enantiocomplementary ; Nitrilase ; Pseudomonas psychrotolerans L19
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2015
- 出版时间：25 December 2015
- 年：2015
- 卷：468
- 期：4
- 页码：820-825
- 全文大小：824 K
文摘
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Four “hot sports” responsible for the nitrilase enantioselectivity were identified.
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The highest S-selective nitrilase L19 mutant toward mandelonitrile was generated.
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A highly R-selective nitrilase L19 mutant with reversed selectivity was generated.
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The changing of enantioselectivity was elucidated by molecular docking experiment.