Solution structure of CEH-37 homeodomain of the nematode Caenorhabditis elegans
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- 作者：Sunjin Moon ; Yong Woo Lee ; Woo Taek Kim ; Weontae Lee
- 关键词：NMR ; nuclear magnetic resonance ; TBP ; telomere-binding protein ; HD ; homeodomain ; C. elegans ; Caenorhabditis elegans ; NOE ; nuclear Overhauser effect ; HSQC ; heteronuclear single quantum coherence
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：10 January, 2014
- 年：2014
- 卷：443
- 期：2
- 页码：370-375
- 全文大小：2045 K
文摘
The nematode Caenorhabditis elegans protein CEH-37 belongs to the paired OTD/OTX family of homeobox-containing homeodomain proteins. CEH-37 shares sequence similarity with homeodomain proteins, although it specifically binds to double-stranded C. elegans telomeric DNA, which is unusual to homeodomain proteins. Here, we report the solution structure of CEH-37 homeodomain and molecular interaction with double-stranded C. elegans telomeric DNA using nuclear magnetic resonance (NMR) spectroscopy. NMR structure shows that CEH-37 homeodomain is composed of a flexible N-terminal region and three 伪-helices with a helix-turn-helix (HTH) DNA binding motif. Data from size-exclusion chromatography and fluorescence spectroscopy reveal that CEH-37 homeodomain interacts strongly with double-stranded C. elegans telomeric DNA. NMR titration experiments identified residues responsible for specific binding to nematode double-stranded telomeric DNA. These results suggest that C. elegans homeodomain protein, CEH-37 could play an important role in telomere function via DNA binding.