Antioxidant activity of hydro distillation water residues from Rosmarinus officinalis L. leaves determined by DPPH assays

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• 作者：Alexander Wollinger^a ; ^{alexander.wollinger@ur.de" class="auth_mail" title="E-mail the corresponding author} ; É ; lodie Perrin^a ; ^{elodie.perrin@hotmail.fr" class="auth_mail" title="E-mail the corresponding author} ; Jamal Chahboun^b ; ^{jamal.chahboun@phytotagante.com" class="auth_mail" title="E-mail the corresponding author} ; Valé ; rie Jeannot^b ; ^{valerie.jeannot@phytotagante.com" class="auth_mail" title="E-mail the corresponding author} ; Didier Touraud^a ; ^{didier.touraud@ur.de" class="auth_mail" title="E-mail the corresponding author} ; Werner Kunz^a ; ^{werner.kunz@ur.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antioxidants ; Rosemary ; Essential oil ; Steam distillation ; Hydro distillation ; DPPH ; Antioxidant activity
• 刊名：Comptes Rendus Chimie
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：19
• 期：6
• 页码：754-765
• 全文大小：1786 K

文摘

Rosemary (Rosmarinus officinalis L.) is a perennial herb with an intensive aromatic flavor. Its most important chemical constituents are essential oils (e.g., 1,8-cineole and camphor) and antioxidants (e.g., carnosic acid and rosmarinic acid). The common methods to extract the essential oil of rosemary are steam or hydro distillation. The aim of this work is to investigate the residual antioxidants after hydro distillation, especially rosmarinic acid and carnosic acid. For this purpose, the hydro distillation water residues were analyzed by HPLC-UV. Moreover, the influence of the extraction duration on the concentration of the antioxidants was investigated. Also, the residual amount of these compounds in the leaves was examined. The total antioxidant activity of the extracts and of the pure compounds was determined by DPPH assays. It is shown that after 2.5 h of hydro distillation the amount of rosmarinic acid and the antioxidant activity in the water residue reaches a maximum value. In addition, the yield and the quality of the essential oil were investigated to draw a comparison between steam and hydro distillations of Moroccan rosemary leaves.