Development of a microarray-based assay for rapid monitoring of genetic variants of West Nile virus circulating in the United States

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• 作者：Andriyan Grinev^a ; ^{Andriyan.Grinev@fda.hhs.gov} ; Caren Chancey^a ; Evgeniya Volkova^a ; Vladimir Chizhikov^b ; Maria Rios^a ; ^{Maria.Rios@fda.hhs.gov}
• 关键词：Flavivirus ; West Nile virus ; Microarray technology ; Viral RNA isolation ; Mutations ; Genetic variability
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：239
• 期：Complete
• 页码：17-25
• 全文大小：1017 K
• 卷排序：239

文摘

A new specific method for isolation of WNV genomic RNA was developed. A microarray-based assay suitable for fast genotyping of WNV was developed. The assay was validated using all WNV genotypes circulating in the US. These new approaches are expected to improve epidemiological surveillance of WNV.