A simple shotgun proteomics method for rapid bacterial identification

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• 作者：Dobryan M. Tracz ; Stuart J. McCorrister ; Patrick M. Chong ; David M. Lee ; Cindi R. Corbett ; Garrett R. Westmacott ; ^{garrett.westmacott@phac-aspc.gc.ca}
• 关键词：MALDI-TOF MS ; matrix-assisted laser desorption-ionization time-of-flight mass spectrometry ; LC-MS/MS ; liquid chromatography tandem mass spectrometry ; BSL-3 ; biosafety level 3 ; BSL-2 ; biosafety level 2
• 刊名：Journal of Microbiological Methods
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：94
• 期：1
• 页码：54-57
• 全文大小：483 K

文摘

Bacterial pathogens were rapidly identified by shotgun proteomics using a novel, easy to implement database search strategy. Peptide sequence data from nano-LC-MS/MS was searched against a database represented by concatenated proteomes of completed genome sequences. Select bacterial species, including BSL-3 select agents, were used to demonstrate this method.