Targeted systemic mesenchymal stem cell delivery using hyaluronate - wheat germ agglutinin conjugate

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• 作者：Yun Seop Kim ; Won Ho Kong ; Hyemin Kim ; Sei Kwang Hahn ; ^{skhanb@postech.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Hyaluronate ; Wheat germ agglutinin ; Mesenchymal stem cell ; Cell surface modification ; Targeted delivery
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：106
• 期：Complete
• 页码：217-227
• 全文大小：3156 K

文摘

A variety of receptors for hyaluronate (HA), a natural linear polysaccharide, were found in the body, which have been exploited as target sites for HA-based drug delivery systems. In this work, mesenchymal stem cells (MSCs) were surface-modified with HA - wheat germ agglutinin (WGA) conjugate for targeted systemic delivery of MSCs to the liver. WGA was conjugated to HA by coupling reaction between aldehyde-modified HA and amine group of WGA. The conjugation of WGA to HA was corroborated by gel permeation chromatography (GPC) and the successful surface modification of MSCs with HA-WGA conjugate was confirmed by confocal microscopy. The synthesized HA-WGA conjugate could be incorporated onto the cellular membrane by agglutinating the cell-associated carbohydrates. Fluorescent imaging for in vivo biodistribution visualized the targeted delivery of the HA-WGA/MSC complex to the liver after intravenous injection. This new strategy for targeted delivery of MSCs using HA-WGA conjugate might be successfully exploited for various regenerative medicines including cell therapy.