Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells
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- 作者：Duncan Baker1 ; 2 ; Adam J. Hirst1 ; Paul J. Gokhale1 ; Miguel A. Juarez3 ; Steve Williams2 ; Mark Wheeler2 ; Kerry Bean2 ; Thomas F. Allison1 ; Harry D. Moore1 ; Peter W. Andrews1 ; Ivana Barbaric1 ; i.barbaric@sheffield.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Human pluripotent stem cells ; genetic changes ; detection methods ; sensitivity
- 刊名：Stem Cell Reports
- 出版年：2016
- 出版时间：8 November 2016
- 年：2016
- 卷：7
- 期：5
- 页码：998-1012
- 全文大小：1204 K
文摘
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hPSCs conform to random sampling rules used for karyotyping
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Excluding mosaicism at <1% level requires sampling >500 metaphases
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qPCR is a rapid assay for detection of commonly amplified regions in hPSCs
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Cultures scored as normal by commonly used methods could harbor up to 10% variants