Cloning and characterization of a riboflavin-binding hexamerin from the larval fat body of a lepidopteran stored grain pest, Corcyra cephalonica

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• 作者：V. Venkat Rao^a ; Thuirei Jacob Ningshen^b ; R.K. Chaitanya^c ; B. Senthilkumaran^a ; Aparna Dutta-Gupta^a ; ^{apdgsl@uohyd.ernet.in" class="auth_mail" title="E-mail the corresponding author} ; ^{aparnaduttagupta@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Fat body ; Hexamerin ; 20-Hydroxyecdysone ; Juvenile hormone ; Riboflavin ; Stored grain pest management
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：April-May 2016
• 年：2016
• 卷：194-195
• 期：Complete
• 页码：58-64
• 全文大小：1989 K

文摘

In the present study, a riboflavin-binding hexamerin (RbHex) was cloned and characterized from the larval fat body of Corcyra cephalonica. The complete cDNA (2121 bp) encodes a 706-amino acid protein with a molecular mass ~ 82 kDa. Expression of RbHex 82 was predominant in fat body among larval tissues. Further, it is prominently expressed during the last instar larval development. Homology modeling and docking studies predicted riboflavin binding site of the hexamerin. Spectrofluorimetric analysis further confirmed riboflavin release from the hexamerin fraction. Quantitative RT-PCR studies demonstrated hormonal regulation of RbHex 82. 20-Hydroxyecdysone (20HE) had a stimulatory effect on its transcription whereas JH alone did not show any effect. However, JH in the presence of 20HE maintains the RbHex 82 expression which indicates the JH's role as a status quo factor. This study is the first to report the characterization of riboflavin-binding hexamerin in a lepidopteran pest. Further, the possibility of RbHex 82 as a pest control target is discussed.