- 作者：Daniel S. Pearl ; Mojgan Masoodi ; Michael Eiden ; Janine Br眉mmer ; Darren Gullick ; Tricia M. Mckeever ; Mark A. Whittaker ; Harriet Nitch-Smith ; James F. Brown ; Janis K. Shute ; Graham Mills ; Philip C. Calder ; Timothy M. Trebble
- 关键词：PUFA ; polyunsaturated fatty acid ; AA ; arachidonic acid ; EPA ; eicosapentaenoic acid ; UC ; ulcerative colitis ; DPA ; docosapentaenoic acid ; DHA ; docosahexaenoic acid ; LA ; linoleic acid ; 伪-LNA ; alpha linolenic acid ; PG ; prostaglandins ; LT ; leukotrienes ; 5-ASA ; 5-aminosalicylic acid ; HPLC ; high performance liquid chromatography ; SPE ; solid phase extraction ; LC/MS ; liquid chromatography mass spectrometry ; EI-SIM ; electron impact selective ion monitoring ; PPAR纬 ; peroxisome proliferator activator recep
- 刊名：Journal of Crohn's and Colitis
- 出版年：1 January, 2014
- 年：2014
- 卷：8
- 期：1
- 页码：70-79
- 全文大小：455 K
Background and Aims
The polyunsaturated fatty acids (PUFA) arachidonic acid (AA, n-6) and eicosapentaenoic acid (EPA, n-3) are precursors of eicosanoids and other lipid mediators which have critical roles in inflammation. The mediators formed from the different PUFA have different potencies. We hypothesised that metabolic changes associated with colonic mucosal inflammation would modify the bioavailability of the eicosanoid precursors AA and EPA.Methods
Colonic mucosa biopsies were obtained from patients with ulcerative colitis and from matched controls. Inflammation was graded endoscopically and histologically. Esterified and non-esterified fatty acids were determined within the biopsies using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively.
Results
Biopsy samples were collected from 69 UC patients (54 providing both inflamed and non-inflamed mucosa) and 69 controls. Inflamed mucosa had higher AA (p < 0.001) and lower EPA (p < 0.010) contents and a higher AA:EPA ratio (p < 0.001). Inflamed mucosa also had higher docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) and lower linoleic acid (LA) and 伪-linolenic acid (伪-LNA) contents (all p < 0.001), compared to non-inflamed and controls. There were significant correlations between severity of inflammation and contents of AA, DPA and DHA (positive correlations) and of LA, 伪-LNA and EPA (negative correlations).
Conclusions
Higher AA, AA:EPA ratio, DPA and DHA and lower LA, 伪-LNA and EPA are seen in inflamed mucosa in UC and correlate with severity of inflammation. This suggests an alteration in fatty acid metabolism in the inflamed gut mucosa, which may offer novel targets for intervention and should be considered if nutritional strategies are used.