Immunogenicity and Specificity of Anti recombinant Protein Fim-C-Salmonella typhimurium Antibody as a Model to Develop Typhoid Vaccine

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• 作者：Muktiningsih Nurjayadi^a ; ^{muktiningsih@unj.ac.id" class="auth_mail" title="E-mail the corresponding author} ; Dea Apriyani^a ; Umar Hasan^a ; Imam Santoso^a ; Fera Kurniadewi^a ; Irma Ratna Kartika^a ; Kurnia Agustini^c ; Fernita Puspasari^b ; Dessy Natalia^b ; Wibowo Mangunwardoyo^d
• 关键词：Salmonella typhimurium ; typhoid fever ; recombinant protein Fim-C-Salmonella typhimurium antibody ; recombinant vaccine
• 刊名：Procedia Chemistry
• 出版年：2016
• 出版时间：2016
• 年：2016
• 卷：18
• 期：Complete
• 页码：237-245
• 全文大小：303 K

文摘

Salmonella typhimurium, that cause typhoid fever in mice was used as a model to study typhoid fever in humans. This study is aimed to determine the immunogenicity of recombinant protein of Fim-C-S. typhimurium and specificity of antibody anti-recombinant protein of Fim-C-S. typhimurium in ddY mice. The immunogenicity studies by ELISA indicated that recombinant protein of Fim-C-S. typhimurium evokes strong immune response in ddY mice, particularly in experiment group. The specificity evaluated by Western Immunobloting technique indicated that the antibody anti-recombinant protein of Fim-C-S. typhimurium can detect the 31 kDa of recombinant protein of Fim-C-S. typhimurium as an antigen. This data reported that recombinant protein of Fim-C-S. typhimurium is immunogenic and the antibody anti-recombinant protein of Fim-C-S. typhimurium is specific. The results of this study can be used as a model to develop recombinant vaccine candidate against typhoid.