A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis
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- 作者：Wichai Soemphola ; b ; Maki Tatsunoa ; Takahiro Okadaa ; Minenosuke Matsutania ; Naoya Kataokaa ; Toshiharu Yakushia ; Kazunobu Matsushitaa ; kazunobu@agr.yamaguchi-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：AAB ; acetic acid bacteria ; AO ; acridine orange ; ISO ; inside&ndash ; out ; YPG ; yeast extract-peptone-glycerol
- 刊名：Journal of Biotechnology
- 出版年：2015
- 出版时间：10 October 2015
- 年：2015
- 卷：211
- 期：Complete
- 页码：46-55
- 全文大小：1869 K
文摘
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Previous data showed an antiporter is involved in thermotolerance in A. tropicalis.
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The gene (At-nhaK2) disruption impaired the growth at high temperatures with pH > 6.5.
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The growth of At-nhaK2-mutant was also shown to be impaired in the high conc. of K+.
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K+/H+ antiporter activity was shown to be defect in the mutantm by the ISO vesicles.
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Phylogenetic analysis showed that At-nhaK2 is a novel type of Na+ /H+ antiporter.