A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle

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• 作者：Afifi Inayah¹ ; Sri Rahayu¹ ; Nashi Widodo¹ ; ^{Widodo@ub.ac.id" class="auth_mail" title="E-mail the corresponding author} ; Widya Ayu Prasdini²
• 关键词：BMP-15 ; Folliculogenesis ; GDF-9 ; In silico ; Docking molecule
• 刊名：Asian Pacific Journal of Reproduction
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：5
• 期：2
• 页码：140-143
• 全文大小：1212 K

文摘

To determine the polymorphism of GDF-9 gene in FH cattle that may affect its interaction with the BMP-15 protein.

Method

Blood was taken from the jugular vein using 10 mL sterile syringes from ten Friesian Holstein cattle. The DNA were isolated from the whole blood and used as a template to amplify GDF-9 gene. The Amplicon (PCR product) was sequenced to identify the new SNP. The three-dimensional structure of GDF-9 protein was modeled by SWISS-MODEL. The characteristic of the protein structure was analyzed by using projectHOPE. The binding affinity GDF-9 into MBP was examined by PatchDock and FireDock.

Results

The result indicates a new variant G1358A changed amino acid residue at position 435 from Arginine into Histidine (R453H). In silico analysis using projectHOPE predicted that the variant altered side chain of GDF-9 and changed its interaction with BMP-15. Further study suggested that the polymorphism R453H also reduce the binding affinity of the GDF-9 into BMP-15 from −85.58 kcal/mol (R453) into −80.79 kcal/mol (H453).

Conclusion

The new variant G1358A at an estrous FH a cattle has potency alter GDF-9 profile that may affect to reproduction performance.