Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis

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• 作者：Yunita Armiyanti¹ ; ^{yunita.fk@unej.ac.id" class="auth_mail" title="E-mail the corresponding author} ; Renam Putra Arifianto² ; Elisa Nurma Riana² ; Kartika Senjarini² ; Widodo Widodo³ ; Loeki Enggar Fitri⁴ ; Teguh Wahju Sardjono⁴
• 关键词：Saliva ; Immunogenic ; Proteins ; Anopheles
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：6
• 期：11
• 页码：924-930
• 全文大小：1156 K

文摘

To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.

Methods

The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography–mass spectrometry.

Results

The proteins that have molecular weight (MW) 43 and 34 kDa were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins (MW: 43 kDa) and two novel hypothetical proteins (MW: 34 kDa). Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi.

Conclusions

The identified proteins are potential to be developed as a biomarker of mosquito bite's exposure.