TP53 and lacZ mutagenesis induced by 3-nitrobenzanthrone in Xpa-deficient human TP53 knock-in mouse embryo fibroblasts
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- 作者：Jill E. Kucaba ; jill.kucab@kcl.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Edwin P. Zwartb ; Harry van Steegb ; Mirjam Luijtenb ; Heinz H. Schmeiserc ; David H. Phillipsa ; Volker M. Arlta
- 关键词：TP53 ; Mutation ; Nucleotide excision repair ; 3-Nitrobenzanthrone ; DNA adduct ; Environmental carcinogen
- 刊名：DNA Repair
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：39
- 期：Complete
- 页码：21-33
- 全文大小：1063 K
文摘
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We examine TP53 and lacZ mutagenesis induced by 3-nitrobenzanthrone (3-NBA).
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We utilize Hupki (Human TP53 knock-in) fibroblasts (HUFs) wild-type and null for Xpa.
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Xpa-Null HUFs are more sensitive to 3-NBA than Xpa-WT HUFs.
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3-NBA induces a similar TP53 and lacZ mutant frequency in Xpa-WT and Xpa-Null HUFs.
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3-NBA induces mutations at TP53 codons that are hotspots in smokers’ lung cancer.