Locked nucleic acid probe enhances Sanger sequencing sensitivity and improves diagnostic accuracy of high-resolution melting-based KRAS mutational analysis
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- 作者：Takayuki Ishigea ; ishige-t@chiba-u.jp" class="auth_mail" title="E-mail the corresponding author ; Sakae Itogaa ; Kazuyuki Matsushitaa ; b ; c ; Fumio Nomurac ; d
- 关键词：EGFR ; epidermal growth factor receptor ; FFPE ; formalin-fixed paraffin-embedded ; HRM ; high-resolution melting ; LNA ; locked nucleic acid ; WT ; wild-type ; MT ; mutant-type ; UNG ; uracil-DNA glycosylase
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：1 June 2016
- 年：2016
- 卷：457
- 期：Complete
- 页码：75-80
- 全文大小：978 K
文摘
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HRM analysis has greater sensitivity than conventional Sanger sequencing.
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LNA probes inhibit wild-type allele synthesis in cycle sequencing.
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The sensitivity of LNA-Sanger sequencing is similar to that of HRM analysis.
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LNA probes improve the diagnostic accuracy of HRM-based KRAS mutational analysis.