Profiling the substitution pattern of xyloglucan derivatives by integrated enzymatic hydrolysis, hydrophilic-interaction liquid chromatography and mass spectrometry
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- 作者：Jun Liua ; Victor Kisonena ; Stefan Willfö ; ra ; Chunlin Xua ; b ; cxu@abo.fi" class="auth_mail" title="E-mail the corresponding author ; Francisco Vilaplanab ; c ; franvila@kth.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Glycomics ; Hemicelluloses ; Mass spectrometry ; Enzymatic hydrolysis ; Oligosaccharide sequencing ; HILIC-ESI&ndash ; MS/MS
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：9 September 2016
- 年：2016
- 卷：1463
- 期：Complete
- 页码：110-120
- 全文大小：1085 K
文摘
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The substitution pattern of xyloglucan (XyG) derivatives is proposed.
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A XyG-specific enzyme selectively releases oligosaccharides for MS analysis.
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Separation of modified XyG oligosaccharides is achieved by HILIC.
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HILIC-ESI–MS/MS allows identification of the substituent position.