Impaired RASGRF1/ERK-mediated GM-CSF response characterizes CARD9 deficiency in French-Canadians

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• 作者：Christina Gavino ; MSc^a ; Nancy Hamel ; MSc^b ; ^c ; Ji Bin Zeng ; PhD^a ; Catherine Legault ; MD^d ; Marie-Christine Guiot ; MD ; PhD^e ; Jeffrey Chankowsky ; MD^f ; Duncan Lejtenyi ; MSc^g ; Martine Lemire ; MD^h ; Isabelle Alarie ; MDⁱ ; Simon Dufresne ; MD^j ; Jean-Nicolas Boursiquot ; MD^k ; Fiona McIntosh ; MSc^l ; Mé ; lanie Langelier ; BScN^a ; Marcel A. Behr ; MD^l ; ^m ; Donald C. Sheppard ; MD^m ; William D. Foulkes ; MBBS ; PhD^b ; ^c ; Donald C. Vinh ; MD^a ; ^c ; ^{donald.vinh@mcgill.ca" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Caspase recruitment domain-containing protein 9 ; spontaneous central nervous system candidiasis ; candidiasis ; Ras protein&ndash ; specific guanine nucleotide-releasing factor 1 ; extracellular signal-regulated kinase ; GM-CSF ; CSF2
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：137
• 期：4
• 页码：1178-1188.e7
• 全文大小：3346 K

文摘

Caspase recruitment domain-containing protein 9 (CARD9) deficiency is an autosomal recessive primary immunodeficiency conferring human susceptibility to invasive fungal disease, including spontaneous central nervous system candidiasis (sCNSc). However, clinical characterization of sCNSc is variable, hindering its recognition. Furthermore, an in-depth understanding of the bases for this susceptibility has remained elusive.

d="absSec_2">Objectives

d="abspara0015">We sought to comprehensively characterize sCNSc and to dissect the mechanisms by which a hypomorphic CARD9 mutation causes susceptibility to Candida species.

d="absSec_3">Methods

d="abspara0020">We describe the clinical and radiologic findings of sCNSc caused by CARD9 deficiency in a French-Canadian cohort. We performed genetic, cellular, and molecular analyses to further decipher its pathophysiology.

d="absSec_4">Results

d="abspara0025">In our French-Canadian series (n = 4) sCNSc had onset in adulthood (median, 38 years) and was often misinterpreted radiologically as brain malignancies; 1 patient had additional novel features (eg, endophthalmitis and osteomyelitis). CARD9 deficiency resulted from a hypomorphic p.Y91H mutation and allelic imbalance established in this population through founder effects. We demonstrate a consistent cellular phenotype of impaired GM-CSF responses. The ability of CARD9 to complex with B-cell CLL/lymphoma 10 (BCL10) and mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is intact in our series, arguing against its involvement in susceptibility to fungi. Instead, we show that the p.Y91H mutation impairs the ability of CARD9 to complex with Ras protein–specific guanine nucleotide-releasing factor 1 (RASGRF1), leading to impaired activation of nuclear factor κB and extracellular signal-regulated kinase (ERK) in monocytes and subsequent GM-CSF responses. Successful treatment of a second patient with adjunctive GM-CSF bolsters the clinical relevance of these findings.

d="absSec_5">Conclusions

d="abspara0030">Hypomorphic CARD9 deficiency caused by p.Y91H results in adult-onset disease with variable penetrance and expressivity. Our findings establish the CARD9/RASGRF1/ERK/GM-CSF axis as critical to the pathophysiology of sCNSc.