Optimizing cyanobacteria growth conditions in a sealed environment to enable chemical inhibition tests with volatile chemicals
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- 作者：Tylor J. Johnson ; tylor.johnson@sdstate.edu" class="auth_mail" title="E-mail the corresponding author ; Jacob D. Zahler ; Emily L. Baldwin ; Ruanbao Zhou ; William R. Gibbons
- 关键词：ANOVA ; analysis of variance ; Chl a ; Chlorophyll α ; MRT ; Duncan's new multiple range test ; U ; fluorescence intensity units
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：126
- 期：Complete
- 页码：54-59
- 全文大小：530 K
文摘
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0.5 g/L NaHCO3 is optimal for cultivating cyanobacteria in a sealed environment.
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Adding NaHCO3 in a fed-batch manner marginally improves growth.
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Sealed environment is necessary to maintain volatile chemicals in solution.
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SYTO® 9 fluorescence assay accurately monitors cyanobacterial growth with minimal sample.