- 作者：Harpreet Johal ; Gillian Marie Scott ; Robert Jones ; Catherine Camaris ; Stephen Riordan ; William D. Rawlinson
- 关键词：Primary Biliary Cirrhosis (PBC) ; p53 tumour suppressor protein ; Estrogen receptor-alpha (ER-α ; ) ; Progesterone receptor (PgR) ; Mouse mammary tumour virus-like virus (MMTV-LV)
- 刊名：Journal of Hepatology
- 出版年：2009
- 出版时间：March 2009
- 年：2009
- 卷：50
- 期：3
- 页码：548-554
- 全文大小：299 K
Background/Aims
The human equivalent of mouse mammary tumour virus (MMTV-LV) may have a role in the pathogenesis of primary biliary cirrhosis (PBC) and breast carcinogenesis. We investigated MMTV-LV prevalence in patients with liver disease of diverse aetiology and associations with hepatic expression of hormonal receptors, p53 protein and complicating hepatocarcinogenesis.Methods
Nested PCR for MMTV-LV envelope was performed using tissue from 210 patients with liver disease and 20 patients with histologically normal liver. Hepatic expression of estrogen receptor-alpha (ER-α), progesterone receptor (PgR) and p53 protein was determined by immunohistochemistry. MMTV-LV expression was also determined in hepatocellular carcinoma (HCC) tissue from 21 patients, in 11 of whom paired non-tumourous liver tissue was available for comparison.
Results
MMTV-LV envelope sequences were present in 25 % (53/210) of liver disease biopsies but not in histologically normal liver (0/20) (P = 0.02). There was no association between MMTV-LV presence and hepatic expression of ER-α or p53 protein. No liver sections were PgR positive. MMTV-LV prevalence was not significantly different in HCC tissue, paired non-tumourous chronic liver disease tissue from the same patients and liver disease tissue from patients without complicating HCC.
Conclusions
Hepatic expression of MMTV-LV is evident in a wide range of non-cirrhotic and cirrhotic liver diseases, irrespective of ER-α, PgR or p53 status, and unrelated to complicating HCC.