Yeast rRNA Expansion Segments: Folding and Function

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• 作者：Lizzette M. Gó ; mez Ramos¹ ; ² ; Johanna M. Smeekens¹ ; Nicholas A. Kovacs¹ ; Jessica C. Bowman¹ ; Roger M. Wartell³ ; Ronghu Wu¹ ; Loren Dean Williams¹ ; ^{loren.williams@chemistry.gatech.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：rRNA ; ribosomal RNA ; ES ; expansion segment ; LSU ; large ribosomal subunit ; ES7 ; expansion segment 7 ; aaRS ; aminoacyl tRNA synthetase ; AspRS ; aspartyl tRNA synthetase ; LysRS ; lysil tRNA synthetase ; TyrRS ; tyrosyl tRNA synthetase ; mRBP ; mRNA-binding protein ; EMSA ; electrophoretic mobility shift assay ; SHAPE ; selective 2&prime ; -hydroxyl acylation analyzed by primer extension ; RT ; reverse transcription
• 刊名：Journal of Molecular Biology
• 出版年：2016
• 出版时间：9 October 2016
• 年：2016
• 卷：428
• 期：20
• 页码：4048-4059
• 全文大小：1360 K

文摘

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d="p0005">Larger rRNAs of complex organisms confer increased functionality to the ribosome.

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d="p0010">Expansion segment 7 (ES7) binding proteins were characterized by RNA pull-down.

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d="p0015">Isolated ES7 is stable and maintains a near-native secondary and tertiary structure.

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d="p0020">ES7 is a binding hub for proteins essential to expanded ribosomal function.

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