C-terminal truncation of rabbit flavin-containing monooxygenase isoform 2 enhances solubility

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• 作者：Sharon K. Krueger ; Lisbeth K. Siddens ; Marilyn C. Henderson ; Jonathan E. VanDyke ; P. Andrew Karplus ; Clifford B. Pereira and David E. Williams
• 关键词：FMO ; Drug metabolism ; Protein solubility ; Protein engineering ; Membrane protein ; Aggregation
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2006
• 出版时间：15 June 2006
• 年：2006
• 卷：450
• 期：2
• 页码：149-156
• 全文大小：230 K

文摘

Flavin-containing monooxygenases (FMO) are membrane-associated enzymes contributing to oxidative metabolism of drugs and other chemicals. There are no known structures similar enough to FMO to provide accurate insights into the structural basis for differences in metabolism observed among FMOs. To develop an FMO amenable to crystallization, we introduced mutations into rabbit FMO2 (rF2) to increase solubility, decrease aggregation, and simplify isolation. Alterations included removal of 26 AA (Δ26) from the carboxyl-terminus, His₆-fusion to the amino-terminus and a double Ser substitution designed to reduce local hydrophobicity. Only Δ26 FMO variants retained normal activity, increased the yield of cytosolic rF2 and decreased protein aggregation. Δ26 constructs increased rF2 in cytosol in low (from 2 to 13 % ), and high salt (from 24 to 62 % ) conditions. His-fusion proteins, while active and useful for purification, did not affect solubility. Δ26 variants should prove useful for identifying conditions suitable for production of an FMO crystal.