Osteopontin is a proximal effector of leptin-mediated non-alcoholic steatohepatitis (NASH) fibrosis
详细信息 查看全文
- 作者：Jason D. Coombesa ; b ; Steve S. Choic ; d ; Marzena Swiderska-Sync ; Paul Mankaa ; e ; Danielle T. Reidf ; Elena Palmab ; g ; Marco A. Briones-Ortaa ; b ; Guanhua Xiec ; Rasha Younisa ; Naoto Kitamuraa ; Marco della Perutag ; Shanna Bitencourth ; Laurent Dollé ; h ; Ye Htun Ooi ; Zhiyong Mij ; Paul C. Kuoj ; Roger Williamsa ; b ; Shilpa Chokshib ; g ; Ali Canbaye ; Lee C. Claridgek ; Bertus Eksteenf ; Anna Mae Diehlc ; Wing-Kin Syna ; b ; j ; l ; m ; wsyn@doctors.org.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：NAFLD ; nonalcoholic fatty liver disease ; OPN ; osteopontin ; TGF-β ; transforming growth factor β ; αSMA ; alpha smooth muscle actin ; MCD diet ; methionine choline deficient diet ; FFPE ; formalin-fixed ; paraffin embedded ; HPF ; high-powered field ; shScr ; short-hairpin RNA against non-target (scrambled) sequence ; shOPN ; short-hairpin RNA against OPN sequence
- 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：1862
- 期：1
- 页码：135-144
- 全文大小：1474 K
文摘
- •
Leptin and Osteopontin are upregulated in NASH and associated with NASH fibrosis
- •
Osteopontin is leptin-regulated and this occurs via the PI3K/Akt signaling pathway
- •
Osteopontin knockdown and neutralization abrogates leptin-mediated fibrogenesis in vitro
- •
OPN neutralization represses diet-induced NASH fibrosis
- •
OPN is a key effector of leptin-mediated fibrogenesis
- •
These data confirm that OPN is an attractive therapeutic target for NASH fibrosis