Betacyanins from Iresine lindenii leaves were separated by HSCCC for the first time.
An orthogonality between the HSCCC and HPLC separation of betacyanins is observed.
Betacyanins of a wide range of polarities were detected in the I. lindenii leaves extract.
The separation of polar betacyanins was enabled at the presence of HFBA ion-pair reagent.
New acylated betacyanins were identified.