Properties of proteins in cancer procoagulant preparations that are detected by anti-tissue factor antibodies

详细信息    查看全文

• 作者：Raasi ; Shari ; Mielicki ; Wojciech P. ; Gordon ; Stuart G. ; Korte ; Wolfgang
• 关键词：Cancer procoagulant ; Tissue factor ; Factor X ; Contamination ; Angiotensinogen precursor ; Vitamin D-binding protein ; α ; -1-Antitrypsin precursor
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2004
• 出版时间：August 15, 2004
• 年：2004
• 卷：428
• 期：2
• 页码：131-135
• 全文大小：224 K

文摘

Cancer procoagulant (CP) and tissue factor (TF; only in complex with Factor VIIa (FVIIa)) can activate FX to FXa. Controversy still exists whether or not CP is an entity different from TF, or whether CP activity is due to contamination of CP preparations with TF/FVIIa complex. We therefore looked for proteins in CP preparations that were detected by anti-TF antibodies and then sequenced these proteins. One- and two-dimensional gels of CP and TF were used to identify proteins immunoreactive to monoclonal anti-CP and anti-TF antibodies (Mabs). Those proteins in the CP preparation recognized by anti-TF antibodies were sequenced. Angiotensinogen precursor, α-1-antitrypsin precursor, and vitamin D-binding protein were identified along with one so far unidentified sequence; however, no TF-sequences were identified. Also, no proteins with the correct molecular weight for TF were identified using anti-TF antibodies. It seems possible that CP preparations contain proteins that have some epitopes similar to the epitopes recognized in TF by anti-TF Mab. However, these proteins do neither have the molecular weight nor the amino acid sequence of TF.