Development of a simple gradient LC–IR interface for the detection of terpenoids from the α-pinene–ozone reaction

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• 作者：Geiger ; Jutta ; Korte ; Ernst-Heiner ; Schrader ; Wolfgang
• 关键词：Interfaces ; LC-IR ; Infrared spectrometry ; Gradient elution ; Terpenoids ; α ; -Pinene ; Ozone
• 刊名：Journal of Chromatography A
• 出版年：2001
• 出版时间：July 13, 2001
• 年：2001
• 卷：922
• 期：1-2
• 页码：99-110
• 全文大小：971 K

文摘

The development of a simple interface between liquid chromatography and infrared spectroscopy (LC–IR) using a coaxial sprayer is described for less volatile analytes. The system consists of a transfer capillary, in which the analytes are transported from the separation column of the gradient-LC to the outlet of the sprayer. This transfer capillary is coaxially surrounded at the outlet by a stainless steel sprayer capillary, which is resistively heated and flushed with nitrogen gas. The samples are sprayed in the manner that the eluent is vaporized by the heated nitrogen when exiting the capillary, while the analytes are deposited on a moving slide made of infrared transparent material (ZnSe or CaF₂). Afterwards the deposited compounds are analyzed with an infrared microscope in transmission. First results from reaction products of the gas phase reaction of α-pinene with ozone are presented.