Screening hydroxynitrile lyases for (R)-pantolactone synthesis
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- 作者：Beate Pscheidt ; Manuela Avi ; Richard Gaisberger ; Franz S. Hartner ; Wolfgang Skranc ; Anton Glieder
- 关键词：Cyanohydrin ; Hydroxynitrile lyase ; HNL ; Prunus sp. ; Hydroxypivalaldehyde ; (R)-Pantolactone
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2008
- 出版时间：June 2008
- 年：2008
- 卷：52-53
- 期：Complete
- 页码：183-188
- 全文大小：317 K
文摘
The application of (R)-hydroxynitrile lyases ((R)-HNLs) enables a simple chemo-enzymatic approach towards (R)-pantolactone synthesis. For the first time, several new recombinant almond (R)-HNL isoenzymes were compared with native HNLs from different Prunus species with respect to cyanohydrin formation from hydroxypivalaldehyde providing the chiral key precursor in HNL based (R)-pantolactone synthesis. Recombinant PaHNL5 (R-selective hydroxynitrile lyase, isoenzyme 5, from Prunus amygdalus) surpasses all other tested natural and recombinant HNL variants. At low pH even very low amounts of crude enzyme catalysed stereoselective hydroxypivalaldehyde cyanohydrin formation in water based reaction systems.