Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii
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- 作者：Woolford ; Alison J. ; Hewinson ; R. Glyn ; Woodward ; Martin ; Dale ; Jeremy W.
- 关键词：Mycobacterium kansasii ; MPB70 antigen ; Bovine tuberculosis
- 刊名：FEMS Microbiology Letters
- 出版年：1997
- 出版时间：March 1, 1997
- 年：1997
- 卷：148
- 期：1
- 页码：43-48
- 全文大小：488 K
文摘
The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.