Activation of formyl peptide receptor like-1 by serum amyloid A induces CCL2 production in human umbilical vein endothelial cells
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- 作者：Ha Young Lee ; Sang Doo Kim ; Jae Woong Shim ; Jeanho Yun ; Koanhoi Kim ; Yoe-Sik Bae
- 关键词：Serum amyloid A ; C– ; C chemokine motif ligand 2 ; Formyl peptide receptor like-1 ; Endothelial cells ; Atherosclerosis
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2009
- 出版时间：6 March 2009
- 年：2009
- 卷：380
- 期：2
- 页码：313-317
- 全文大小：323 K
文摘
We investigated the effects of serum amyloid A (SAA) on the production of C–C chemokine motif ligand 2 (CCL2) and the mechanism underlying SAA action in human umbilical vein endothelial cells (HUVECs). Stimulation of HUVECs by SAA elicited CCL2 production in a concentration-dependent manner. SAA induced the activations of NF-κB and AP-1, which were essential for CCL2 production after SAA stimulation. HUVECs expressed formyl peptide receptor-like 1 (FPRL1), and short interfering RNA knockdown of FPRL1 nearly completely blocked SAA-induced CCL2 production in HUVECs. We suggest that SAA stimulates CCL2 production via FPRL1 and, thus, contributes to atherosclerosis.