Protective effects of carnosine against malondialdehyde-induced toxicity towards cultured rat brain endothelial cells
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- 作者：Hipkiss ; Alan R. ; Preston ; Jane E. ; Himswoth ; David T.M. ; Worthington ; Viki C. ; Abbot ; N. Joan
- 关键词：Carnosine ; Malondialdehyde ; Reactive oxygen species ; Vascular endothelial cells ; Protein cross-links and carbonyl groups ; 3-[4 ; 5-Dimethylthiazol-2-yl]&ndash ; 2 ; 5-diphenyltetrazolium bromide assay
- 刊名：Neuroscience Letters
- 出版年：1997
- 出版时间：December 5, 1997
- 年：1997
- 卷：238
- 期：3
- 页码：135-138
- 全文大小：412 K
文摘
Malondialdehyde (MDA) is a deleterious end-product of lipid peroxidation. The naturally-occurring dipeptide carnosine (β-alanyl--histidine) is found in brain and innervated tissues at concentrations up to 20 mM. Recent studies have shown that carnosine can protect proteins against cross-linking mediated by aldehyde-containing sugars and glycolytic intermediates. Here we have investigated whether carnosine is protective against malondialdehyde-induced protein damage and cellular toxicity. The results show that carnosine can (1) protect cultured rat brain endothelial cells against MDA-induced toxicity and (2) inhibit MDA-induced protein modification (formation of cross-links and carbonyl groups).