In vitro oxidative activity of cupric complexes of kanamycin A in comparison to in vivo bactericidal efficacy

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• 作者：Szczepanik ; Wojciech ; Dworniczek ; Ewa ; Ciesiolka ; Jerzy ; Wrzesinski ; Jan ; Skala ; Jacek ; Jezowska-Bojczuk ; Malgorzata
• 关键词：Kanamycin A ; Copper(II) complexes ; 2&prime ; -Deoxyguanosine oxidation ; DNA and RNA cleavage ; Bactericidal efficiency
• 刊名：Journal of Inorganic Biochemistry
• 出版年：2003
• 出版时间：April 1, 2003
• 年：2003
• 卷：94
• 期：4
• 页码：355-364
• 全文大小：709 K

文摘

The interactions of copper(II) complexes of kanamycin A with oxidation-susceptible biomolecules: 2′-deoxyguanosine, plasmid DNA and yeast tRNA^Phe were studied in both the presence and absence of hydrogen peroxide. The mixture of complex with H₂O₂ was found to be an efficient oxidant, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNA^Phe. Some of these reactions may play a role in toxic effects of aminoglycoside antibiotics. These complexes were screened for their antibacterial activity. The microbiological studies undertaken to compare the bactericidal action of kanamycin A alone and complexed with copper(II) ions in both neutral and oxidative environment revealed that the enhancement of bactericidal action by Cu(II) was not statistically significant.