An enhanced chemiluminescence resonance energy transfer aptasensor based on rolling circle amplification and WS2 nanosheet for Staphylococcus aureus detection
Co2+ enhanced ABEI functional flowerlike gold nanoparticles (Co2+/ABEI-AuNFs) was first used as chemiluminescent donor. Rolling circle amplification (RCA) technique was also applied in the WS2 nanosheet based CRET system for the first time. The improved WS2 nanosheet based CRET platform can overcome the limitation that only DNA or RNA can be detected and expand the detection scope. A steady-state CRET aptasensor based on P-Iodophenol (PIP) and RCA was fabricated to detect S. aureus with high sensitivity and specificity.