IL-18 accelerates the cell apoptosis by up-regulating Cysteinyl Leukotriene 2 Receptor Expression in Human Umbilical Vein Endothelial Cells at the early stage of administration

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• 作者：Guangyi Zhou ; Zhiming Zhou ; Song Ge ; Dezhi Liu ; Renliang Zhang ; Gelin Xu ; Wusheng Zhu ; Qin Yin ; Alex F. Chen ; Xinfeng Liu
• 关键词：Interleukin-18 ; Receptor ; Leukotrienes ; Endothelial cells ; Apoptosis
• 刊名：Vascular Pharmacology
• 出版年：2009
• 出版时间：May-June 2009
• 年：2009
• 卷：50
• 期：5-6
• 页码：171-177
• 全文大小：1038 K

文摘

The purpose of the present study is to identify whether interleukin (IL)-18 can modulate cysteinyl leukotriene 2 receptor (CysLT₂R) expression in Human Umbilical Vein Endothelial Cells (HUVECs) and how it influences the cell death. According to the results from real-time reverse transcription PCR, confocal laser scanning microscopy, and western blotting, a dose-dependent augmentation of CysLT₂R protein expression in HUVECs was triggered by IL-18 for the first 2 h followed by down-regulation within the next 22 h after IL-18 administration. The flow cytometry showed that non-selective CysLT₁R and CysLT₂R antagonist BAY-u9773 could attenuate the early stage apoptosis mediated by IL-18 whereas CysLT₁R antagonist Montelukast couldn't. Also, pretreatment with BAY-u9773 suppressed calcium influx of HUVECs induced by IL-18 whereas Montelukast didn't work. The observation that progression of cell death aggravated by IL-18 could be attenuated by BAY-u9773 may offer a chance to develop a novel way to treat arteriosclerosis.