Construction and characterization of HIV type 1 CRF07_BC infectious molecular clone from men who have sex with men
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- 作者：Yan-Ling Jianga ; Wen-Wei Baib ; Fan-Wei Quc ; Hua Mad ; Run-Sheng Jiange ; rs_jiang2015@126.com" class="auth_mail" title="E-mail the corresponding author ; Bao-Sheng Shene
- 关键词：HIV-1 ; CRF07_BC ; Men who have sex with men ; Infectious molecular clone ; Full-length cDNA clone ; Long terminal repeat
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：229
- 期：Complete
- 页码：70-77
- 全文大小：2234 K
文摘
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A total of 186 full-length molecular clones of HIV-1CRF07_BC were constructed.
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CRF07_BC subtype is one of the most prevalent HIV-1 strains in MSM population.
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All clones from 4 samples, HNXX1302/1304/1305/1306, were not able to infect human PBMCs.
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Human PBMCs could be infected by the chimeric full-length clones of 1306N6/N13/N22.
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pNL4-3/07BCLTR may significantly improve the in vitro infectivity of CRF07_BC strain.