The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro

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• 作者：Zhiyong Liu ; Andrew B. West ; ^{abwest@uab.edu}
• 关键词：PARK8 ; ROCO4 ; Dardarin ; Enzyme kinetics ; Manganese ; Nucleotide-binding pocket
• 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1865
• 期：3
• 页码：274-280
• 全文大小：972 K
• 卷排序：1865

文摘

The LRRK2 kinase domain can use GTP for both autophosphorylation and trans peptide phosphorylation activity. The pathological mutation G2019S increased GTP-dependent kinase activity. LRRK2 utilization of GTP as a phospho-donor changes the preferences of LRRK2 autophosphorylation.