Physicochemical and biological properties of 2-O-α-d-galactosyl-cyclomaltohexaose (α-cyclodexterin) and -cyclomaltoheptaose (β-cyclodextrin)

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• 作者：Yasuyo Okada ; ^a ; ^{okada@mukogawa-u.ac.jp"" rel=""nofollow} ; Masanori Semma^a ; Atsushi Ichikawa^a
• 关键词：Branched cyclodextrin ; 2-O-&#x3b1 ; -d-Galactosyl-&#x3b1 ; -cyclodextrin ; 2-O-&#x3b1 ; -d-Galactosyl-β ; -cyclodextrin ; Inclusion complex ; Hemolytic activity ; Cytotoxicity
• 刊名：Carbohydrate Research
• 出版年：2011
• 出版时间：1 June 2011
• 年：2011
• 卷：346
• 期：8
• 页码：1018-1022
• 全文大小：547 K

文摘

The physicochemical and biological properties of the new branched cyclomaltooligosaccharides (cyclodextrins; CDs), 2-O-α-d-galactosyl-cyclomaltohexaose (2-O-α-d-galactosyl-α-cyclodextrin, 2-Gal-αCD) and 2-O-α-d-galactosyl-cyclomaltoheptaose (2-O-α-d-galactosyl-β-cyclodextrin, 2-Gal-βCD), were investigated. The formation of inclusion complexes of 2-Gal-CDs with various kinds of guest compounds (clofibrate, cholesterol, cholecalciferol, digitoxin, digitoxigenin, and prostaglandin A₁) was examined by a solubility method, and the results were compared with those of non-branched CDs and other 6-O-glycosyl-CDs such as 6-O-α-d-galactosyl-CDs, 6-O-α-d-glucosyl-CDs, and 6-O-α-maltosyl-CDs. The inclusion abilities of 2-Gal-αCD for clofibrate and prostaglandin A₁, and 2-Gal-βCD for clofibrate, cholecalciferol, cholesterol, and digitoxigenin were markedly weaker than those of non-branched CD and other 6-O-glycosyl-CDs in each series, probably because of a steric hindrance caused by the α-(1→2)-galactoside linkage. The hemolytic activities of 2-Gal-CDs on human erythrocytes were the lowest among each CD series, and the compounds showed negligible cytotoxicity towards Caco-2 cells up to at least 100 mM.