A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

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• 作者：Guido M. Voets^a ; ^{g.voets@umcutrecht.nl} ; A.C. Fluit^a ; Jelle Scharringa^a ; James Cohen Stuart^a ; Maurine A. Leverstein-van Hall^a ; ^b
• 关键词：Multiplex PCR ; Extended-spectrum &#x3b2 ; -lactamases ; Plasmid-mediated AmpC &#x3b2 ; -lactamases ; OXA &#x3b2 ; -lactamases ; Carbapenemases
• 刊名：International Journal of Antimicrobial Agents
• 出版年：2011
• 出版时间：April 2011
• 年：2011
• 卷：37
• 期：4
• 页码：356-359
• 全文大小：291 K

文摘

Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.