Human brain cells used to compare neurotoxicity to cytotoxicity for anticancer drugs.
Neurons stained for βIII-tubulin, astrocytes for GFAP, all nuclei (DAPI) in monolayer
Topoisomerase II drugs were 5–10 times less toxic to neurons compared to all cells.
Levels of neuronal and glial marker proteins in 3D confirmed lower neurotoxicity.