Preparative isolation of osthol and xanthotoxol from Common Cnidium Fruit (Chinese traditional herb) using stepwise elution by high-speed counter-current chromatography

详细信息    查看全文

• 作者：Wei ; Yun ; Zhang ; Tianyou ; Ito ; Yoichiro
• 关键词：Counter-current chromatography ; Preparative chromatography ; Pharmaceutical analysis ; Cnidium monnieri ; Osthol ; Xanthotoxol
• 刊名：Journal of Chromatography A
• 出版年：2004
• 出版时间：April 16, 2004
• 年：2004
• 卷：1033
• 期：2
• 页码：373-377
• 全文大小：139 K

文摘

Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of osthol and xanthotoxol from Cnidium monnieri (L.) Cusson (Common Cnidium Fruit) using stepwise elution with a pair of two-phase solvent systems composed of n-hexane–ethyl acetate–methanol–water at (1:1:1:1, v/v), and (5:5:6:4, v/v), which had been selected by analytical high-speed counter-current chromatography. Using a preparative unit of the HSCCC centrifuge, about a 308mg amount of the crude extract was separated, yielding 88.3mg of osthol and 19.4mg of xanthotoxol at a high purity of over 98 % .