Exposure of cultured fibroblasts to the peptide PR-11 for the identification of induced proteome alterations and discovery of novel potential ligands
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- 作者：Gustavo Silveira Bregueza ; Leandro Xavier Nevesb ; Karina Taciana Santos Silvac ; Lorran Miranda Andrade de Freitasa ; Gabriela de Oliveira Fariaa ; Mauro Cé ; sar Isoldid ; William Castro-Borgesd ; Milton Hé ; rcules Guerra de Andraded ; miltonguerra00@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：PR-39 ; PR-11 ; Proline rich-peptides ; Label-free shotgun ; PR-11 binding proteins
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：1864
- 期：12
- 页码：1775-1786
- 全文大小：910 K
文摘
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Alterations caused by PR-11 on fibroblast culture were revealed by shotgun proteomics.
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Signalling and transcription/translation represents over 50% of altered proteins.
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Proteins associated to regulation of the HIF-1α and NF-κB pathways were detected.
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The gC1qR protein was identified as a ligand of PR-11 by affinity chromatography.