Cytoskeleton dynamics: Fluctuations within the network

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• 作者：Predrag Bursac ; Ben Fabry ; Xavier Trepat ; Guillaume Lenorm ; James P. Butler ; Ning Wang ; Jeffrey J. Fredberg ; Steven S. An
• 关键词：Cytoskeleton ; Remodeling ; Lipid dynamics ; Airway smooth muscle
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：6 April 2007
• 年：2007
• 卷：355
• 期：2
• 页码：324-330
• 全文大小：294 K

文摘

Out-of-equilibrium systems, such as the dynamics of a living cytoskeleton (CSK), are inherently noisy with fluctuations arising from the stochastic nature of the underlying biochemical and molecular events. Recently, such fluctuations within the cell were characterized by observing spontaneous nano-scale motions of an RGD-coated microbead bound to the cell surface [Bursac et al., Nat. Mater. 4 (2005) 557–561]. While these reported anomalous bead motions represent a molecular level reorganization (remodeling) of microstructures in contact with the bead, a precise nature of these cytoskeletal constituents and forces that drive their remodeling dynamics are largely unclear. Here, we focused upon spontaneous motions of an RGD-coated bead and, in particular, assessed to what extent these motions are attributable to (i) bulk cell movement (cell crawling), (ii) dynamics of focal adhesions, (iii) dynamics of lipid membrane, and/or (iv) dynamics of the underlying actin CSK driven by myosin motors.