Modulation of aryl hydrocarbon receptor target genes in circulating lymphocytes from dairy cows bred in a dioxin-like PCB contaminated area

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• 作者：Flavia Girolami^a ; ^{flavia.girolami@unito.it} ; Veronica Spalenza^b ; ^{veronica.spalenza@unito.it} ; Monica Carletti^a ; ^{monica.carletti@unito.it} ; Paola Sacchi^b ; ^{paola.sacchi@unito.it} ; Roberto Rasero^b ; ^{roberto.rasero@unito.it} ; Carlo Nebbia^a ; ^{carlo.nebbia@unito.it}
• 关键词：PCDDs ; polychlorodibenzo-p-dioxins ; PCDFs ; polychlorodibenzofurans ; PCBs ; polychlorobiphenyls ; DL ; dioxin-like ; AHR ; aryl hydrocarbon receptor ; ARNT ; AHR nuclear translocator ; XREs ; xenobiotic-response elements ; AHRR ; AHR repressor ; TCDD ; 2 ; 3 ; 7 ; 8 tetrachl
• 刊名：Science of the Total Environment
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：450-451
• 期：Complete
• 页码：7-12
• 全文大小：374 K

文摘

Animal productions (i.e. fish, eggs, milk and dairy products) represent the major source of exposure to dioxins, furans, and dioxin-like (DL) polychlorobiphenyls for humans. The negative effects of these highly toxic and persistent pollutants are mediated by the activation of the aryl hydrocarbon receptor (AHR) that elicits the transcriptional induction of several genes, including those involved in xenobiotic metabolism. Previously we demonstrated the presence and functioning of the AHR signaling pathway in primary cultures of bovine blood lymphocytes. The aim of the present study was to investigate by real time PCR the expression and the inducibility of selected target genes (i.e. AHR, AHR nuclear translocator (ARNT), AHR repressor, CYP1A1 and CYP1B1) in uncultured cells from dairy cows naturally exposed to DL-compounds. The study was carried out on two groups of animals bred in a highly polluted area and characterized by a different degree of contamination, as assessed by bulk milk TEQ values, and a control group reared in an industry free area. Bovine lymphocytes expressed only AHR, ARNT and CYP1B1 genes to a detectable level; moreover, only CYP1B1 expression appeared to be correlated to TEQ values, being higher in the most contaminated group, and decreasing along with animal decontamination. Finally, lymphocytes from exposed cows displayed a lower inducibility of both CYP1A1 and CYP1B1 after the in vitro treatment with a specific AHR ligand. In conclusion, our results indicate that DL-compound contaminated cows may display significant changes in AHR-target gene expression of circulating lymphocytes.