文摘
As part of our ongoing search for bioactive constituents of natural Korean medicinal plants, three new lignan derivatives, sambucasinol A–C (class="boldFont">1–class="boldFont">3), together with 7 known compounds (class="boldFont">4–class="boldFont">10) were isolated from the twigs of Sambucus williamsii. The structures of these new compounds were determined by a combination of 1D and 2D NMR spectroscopic data analysis, as well as circular dichroism (CD) spectroscopy studies. Here, we evaluated the anti-inflammatory effects of class="boldFont">1–class="boldFont">10 in lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cells. Compounds class="boldFont">1–class="boldFont">3 exhibited significant inhibitory effects on nitric oxide production in LPS-activated BV-2 cells, with IC50 values of 6.82, 7.04, and 14.70 μM, respectively. Additionally, we evaluated the effects of compounds class="boldFont">1–class="boldFont">10 on NGF induction in a C6 rat glioma cell line. Compounds class="boldFont">1–class="boldFont">3 upregulated NGF secretion to 183.95 ± 2.63%, 153.99 ± 5.15%, and 155.96 ± 5.15%, respectively, without any significant cell toxicity. Moreover, all isolates were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and XF498 cell lines. Compounds class="boldFont">1–class="boldFont">3 showed consistent cytotoxicity against the four human cell lines, with IC50 values in the range of 11.07–19.62 μM.