A simple and robust approach to immobilization of antibody fragments
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- 作者：Svetlana P. Ikonomova ; Ziming He ; Amy J. Karlsson ; ajkarl@umd.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：scFv ; single-chain variable fragment ; 6XHis ; hexahistidine ; BCCP ; biotin carboxyl carrier protein ; gpD ; bacteriophage lambda head protein D ; IPTG ; isopropyl β-d-1-thiogalactopyranoside ; HRP ; horseradish peroxidase
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：435
- 期：Complete
- 页码：7-16
- 全文大小：1695 K
文摘
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E. coli cells site-specifically biotinylated scFvs in vivo during expression.
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scFvs were immobilized via the high-affinity biotin-streptavidin interaction.
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Immobilization of scFvs was achieved directly from cell lysate with no purification.
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Biotinylation efficiency and biotinylation tag affected immobilization.
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Immobilized scFvs bound antigen and retained this ability for at least 100 days.