E. coli cells site-specifically biotinylated scFvs in vivo during expression.
scFvs were immobilized via the high-affinity biotin-streptavidin interaction.
Immobilization of scFvs was achieved directly from cell lysate with no purification.
Biotinylation efficiency and biotinylation tag affected immobilization.
Immobilized scFvs bound antigen and retained this ability for at least 100 days.