Acetylcholinesterase inhibitory-active components of Rhodiola rosea L.
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- 作者：Hua Wang ; Gaochao Zhou ; Xiangdong Gao ; Yidan Wang ; Wenbing Yao
- 关键词：Rhodiola rosea L. ; Acetylcholinesterase ; Anti-cholinesterase activity ; Hydroquinone
- 刊名：Food Chemistry
- 出版年：2007
- 出版时间：2007
- 年：2007
- 卷：105
- 期：1
- 页码：24-27
- 全文大小：150 K
文摘
An activity-directed fractionation and purification process was used to identify the acetylcholinesterase inhibitory-active components of Rhodiola rosea L. (RR). Dried rhizome of RR was extracted with boiled ethanol. After removal of tannins, the extract was separated into chloroform, ethyl acetate, n-butanol and water fractions. Among these, chloroform and n-butanol fractions showed stronger activity by bioassay for anti-cholinesterase activity than did ethyl acetate and water fractions. The chloroform fraction was then subjected to separation and purification using silica gel column chromatography and Sephadex LH-20 chromatography. One compound, showing strong anti-cholinesterase activity, was identified by spectral methods (NMR, UV and MS) and by comparison with authentic samples. It proved to be hydroquinone.