Cantharidin reverses multidrug resistance of human hepatoma HepG2/ADM cells via down-regulation of P-glycoprotein expression
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- 作者：Li Hua Zheng ; Yong Li Bao ; Yin Wu ; Chun Lei Yu ; XiangYing Meng ; Yu Xin Li
- 关键词：Multi drug resistance ; P-glycoprotein ; Cantharidin
- 刊名：Cancer Letters
- 出版年：2008
- 出版时间：8 December 2008
- 年：2008
- 卷：272
- 期：1
- 页码：102-109
- 全文大小：279 K
文摘
Multidrug resistance (MDR) is a serious obstacle encountered in cancer treatment. In this study, we established an in vitro multiple drug resistant HepG2 cell line (HepG2/ADM), and characterized its MDR. This model was used to screen potential candidate chemosensitisers from over 200 purified naturally occurring compounds extracted from plants and animals. Cantharidin was found to have a significant reversal on MDR in our model. Further, our results showed that Cantharidin could significantly inhibit P-gp (P-glycoprotein) expression, mRNA transcription, as well as MDR1 promoter activity. These results suggest that Cantharidin is a novel and potent MDR reversal agent and may be a potential adjunctive agent for tumor chemotherapy.