Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods

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• 作者：Xiangzhao Mao ; Yaling Shen ; Liang Yang ; Shi Chen ; Yuping Yang ; Jingya Yang ; Hu Zhu ; Zixin Deng ; Dongzhi Wei
• 关键词：Novel antibiotic ; FR-008/Candicidin derivatives ; Optimization ; Plackett– ; Burman design ; Response surface methodology ; Enzymatic pathways
• 刊名：Process Biochemistry
• 出版年：2007
• 出版时间：May 2007
• 年：2007
• 卷：42
• 期：5
• 页码：878-883
• 全文大小：275 K

文摘

Medium compositions for the production of the novel derivatives of FR-008/Candicidin which was produced by a mutant of Streptomyces sp. FR-008 were optimized using two statistical methods including Plackett–Burman design (P–B), which was applied to find the key ingredients for the best medium composition, and response surface methodology (RSM), which was used to determine the optimal concentrations of these components. Results indicated that peptone, copper sulfate and glycerol had significant effects on the production. Under the proposed optimized conditions, the CS101 experimental yield (191.259 mg/L) closely matched the yield (203.536 mg/L) predicted by the statistical model. The optimization of the medium contributed to 10-fold higher antibiotic production than that of the control. It was first revealed that copper could stimulate FR-008/Candicidin and their derivatives synthesis at an optimal concentration in this paper, moreover, the basis of this phenomenon was also explained by investigating the enhancement of the enzymatic pathways.