Synthesis of diamine maleyl chitosans, and in vitro transfection studies

详细信息    查看全文

• 作者：Bo  ; Lu^a ; Xu  ; Liu^a ; Zhijun  ; Huang^a ; Haixing  ; Xu^a ; Peihu  ; Xu^a ; Yiting  ; Wang^a ; Hua  ; Zheng^a ;   ; ^{whutlvb@163.com} ; Yihua  ; Yin^a ; Xianzheng  ; Zhang^b ;   ; ^{whutyl@163.com} ; Renxi  ; Zhuo^b
• 关键词：Chitosan ; Gene vector ; Transfection efficiency ; Non-viral vector
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 出版时间：15 January 2012
• 年：2012
• 卷：87
• 期：2
• 页码：1453-1459
• 全文大小：931 K

文摘

Three novel diamine-modified chitosan derivatives were synthesized from N-maleyl chitosan via Michael addition reaction with 1,2-diaminoethane, 1,4-diaminobutane, and 1,6-diaminohexane, respectively. These chitosan derivatives exhibited well binding ability of condensing plasmid DNA to form complexes with size ranging from 150 to 500 nm when the chitosan derivative/DNA weight ratios were above 10. The complexes observed by scanning electron microscopy (SEM) exhibited a compact and spherical morphology. The cytotoxicity of the chitosan derivatives presented a dependence on their side-chain structures. The gene transfection experiments were evaluated in 293 T and HeLa cells. The data obtained demonstrated that the gene transfection efficiencies of these chitosan derivatives were better than that of chitosan, suggesting these chitosan derivatives as potential gene vectors in vitro.