Neuroprotective effect and mechanism of Mu-Xiang-You-Fang on cerebral ischemia-reperfusion injury in rats

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• 作者：Qipeng Zhao^a ; ^b ; ¹ ; Xiuli Cheng^a ; ¹ ; Xiaobo Wang^a ; Jing Wang^a ; ^b ; Yafei Zhu^a ; ^{867453132@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Xueqin Ma^a ; ^b ; ^{maxueqin217@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Methyleugenol (PubChem CID ; 7127) ; Caryophyllene (PubChem CID ; 5281515) ; Myristicin (PubChem CID ; 4276) ; Methyl arachidonate (PubChem CID ; 6421258) ; Costunolide (PubChem CID ; 5281437) ; Dehydrocostus lactone (PubChem CID ; 73174) ; Piperine (PubChem CID ; 638024) ; Sesamin(PubChem CID ; 72303) ; Tris base (PubChem CID ; 6503) ; SDS (PubChem CID ; 3423265)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 November 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：140-147
• 全文大小：3558 K

文摘

The present study is to investigate the neuroprotective effect of Mu-Xiang-You-Fang (MXYF), a classic Traditional Chinese Medicine used by Chinese minorities to treat stroke, on cerebral ischemia-reperfusion (I/R) injury and the related signaling pathways.

Materials and methods

Male Sprague-Dawley rats were divided into 6 groups: sham group, I/R group, nimodipine and MXYF (58, 116 and 232 mg/kg respectively) groups. Cerebral ischemia model was induced by middle cerebral artery occlusion for 2 h followed by reperfusion for 48 h. Neurological functional score was evaluated according to the method of Zea longa’s score and the infarct area was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining at 48 h after reperfusion. The protein expression of cytochrome c (cyt-c), Bcl-2, Bax, caspase-9, caspase-3 and caspase-7 were analyzed by western blot and the mRNA expression of Caspase-9, Caspase-3 and Caspase-7 were determined by the reverse transcription-polymerase chain reaction.

Results

Oral administration of MXYF (116 and 232 mg/kg) significantly reduced the neurological functional score and attenuated the cerebral infarct area. Western blot analysis showed that the expression of Bcl-2 is enhanced and Bax expression is inhibited after treatment with MXYF (116 and 232 mg/kg), leading to significant increase of the ratio between Bcl-2 and Bax. Furthermore, the protein expression of cyt-c, caspase-9, caspase-3 and caspase-7 was significantly inhibited while the mRNA expression of caspase-9, caspase-3 and caspase-7 but not cyt-c was markedly inhibited in the MXYF (116 and 232 mg/kg) treatment groups compared with the I/R group.

Conclusions

The above data suggested that MXYF has potential neuroprotective activities by the regulation of apoptotic pathway, MXYF is a promising agent in treatment of stroke.