Two-step purification of low-content cellular protein using protein-imprinted polymers

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• 作者：Yi Long ; Xiaocui Xing ; Ruifang Han ; Yang Sun ; Ying Wang ; Zhuo Zhao ; Huaifeng Mi
• 关键词：Protein-imprinted polymer ; Assistant recognition polymer chains (ARPCs) ; Purification of cellular protein ; Cloned bacterial protein as template
• 刊名：Analytical Biochemistry
• 出版年：2008
• 出版时间：15 September 2008
• 年：2008
• 卷：380
• 期：2
• 页码：268-275
• 全文大小：1055 K

文摘

We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.