Simultaneous detection of 13 viruses involved in meningoencephalitis using a newly developed multiplex PCR Mag-array system
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- 作者：Xiaodan Shia ; 1 ; Rui Wua ; 1 ; Ming Shia ; Linfu Zhoua ; Mengli Wua ; Yining Yanga ; Xinyue Ana ; Wen Daia ; Liang Tiana ; Chen Zhangb ; Xuejun Mab ; maxj@ivdc.chinacdc.cn" class="auth_mail" title="E-mail the corresponding author ; Gang Zhaoa ; zhaogang@fmmu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：MPMA system ; Meningoencephalitis viruses ; Cerebrospinal fluid
- 刊名：International Journal of Infectious Diseases
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：49
- 期：Complete
- 页码：80-86
- 全文大小：511 K
文摘
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Current methods for pathogen detection in central nervous system (CNS) viral infection are limited.
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The multiplex PCR Mag-array (MPMA) system detects 13 CNS disorder related viruses.
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The MPMA system showed high specificity and efficiency in control plasmid detection.
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MPMA cerebrospinal fluid testing identified pathogens in 20% of undiagnosed samples.
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Sensitivity and specificity for detecting herpes simplex virus (HSV)-1/2 were 100% and >98%, respectively.